| Host: | Mouse |
| Applications: | WB/ICC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Cdc42 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | M430 |
| Conjugation: | Unconjugated |
| Isotype: | IgM |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:500IHCICC 1:200IP |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Cdc42 |
| Gene ID: | 64465 |
| Uniprot ID: | CDC42_RAT |
| Specificity: | The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein. |
| Immunogen: | Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42. |
| Post Translational Modifications | Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups. |
| Protein Name | Cell Division Control Protein 42 Homolog |
| Database Links | Reactome: R-RNO-114604Reactome: -RNO-182971Reactome: -RNO-2029482Reactome: -RNO-389359Reactome: -RNO-3928662Reactome: -RNO-418885Reactome: -RNO-4420097Reactome: -RNO-525793Reactome: -RNO-5625970Reactome: -RNO-5626467Reactome: -RNO-5627123Reactome: -RNO-5663213Reactome: -RNO-5663220Reactome: -RNO-5687128Reactome: -RNO-8964616Reactome: -RNO-9013148Reactome: -RNO-9013149Reactome: -RNO-9013404Reactome: -RNO-9013406Reactome: -RNO-9013408Reactome: -RNO-9013420Reactome: -RNO-9013423Reactome: -RNO-9013424Reactome: -RNO-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideMidbodyCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleCell ProjectionLamellipodium MembranePeripheral Membrane ProteinDendriteLocalizes To Spindle During Prometaphase CellsMoves To The Central Spindle As Cells Progressed Through Anaphase To TelophaseLocalizes At The End Of Cytokinesis In The Intercellular Bridge Formed Between Two Daughter CellsIts Localization Is Regulated By The Activities Of Guanine Nucleotide Exchange Factor Ect2 And Gtpase Activating Protein Racgap1Colocalizes With Nek6 In The CentrosomeIn Its Active Gtp-Bound Form Localizes To The Leading Edge Membrane Of Migrating Dendritic Cells |
| Alternative Antibody Names | Anti-Cell Division Control Protein 42 Homolog antibodyAnti-Cdc42 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
