• Western blot analysis of various lysates, using CDA antibody (STJ115894) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using CDA antibody (STJ115894) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CDA Antibody (STJ115894) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using CDA antibody (STJ115894). Blue: DAPI for nuclear staining.

Anti-CDA antibody (1-146) (STJ115894)

SKU:
STJ115894

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDA (1-146) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDA
Gene ID: 978
Uniprot ID: CDD_HUMAN
Immunogen Region: 1-146
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CDA (NP_001776.1).
Immunogen Sequence: MAQKRPACTLKPECVQQLLV CSQEAKKSAYCPYSHFPVGA ALLTQEGRIFKGCNIENACY PLGICAERTAIQKAVSEGYK DFRAIAIASDMQDDFISPCG ACRQVMREFGTNWPVYMTKP DGTYIVMTVQELLPSSFGPE DLQKTQ
Tissue Specificity Highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines.
Function This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Protein Name Cytidine Deaminase
Cytidine Aminohydrolase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-73614
Alternative Antibody Names Anti-Cytidine Deaminase antibody
Anti-Cytidine Aminohydrolase antibody
Anti-CDA antibody
Anti-CDD antibody

Information sourced from Uniprot.org

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