• Western blot analysis of extracts of various cell lines, using CD83 antibody (STJ23013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD83 antibody (20-205) (STJ23013)

SKU:
STJ23013

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD83 (20-205) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD83
Gene ID: 9308
Uniprot ID: CD83_HUMAN
Immunogen Region: 20-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-205 of human CD83 (NP_004224.1).
Immunogen Sequence: TPEVKVACSEDVDLPCTAPW DPQVPYTVSWVKLLEGGEER METPQEDHLRGQHYHQKGQN GSFDAPNERPYSLKIRNTTS CNSGTYRCTLQDPDGQRNLS GKVILRVTGCPAQRKEETFK KYRA
Tissue Specificity Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
Function May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Protein Name Cd83 Antigen
Hcd83
B-Cell Activation Protein
Cell Surface Protein Hb15
Cd Antigen Cd83
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd83 Antigen antibody
Anti-Hcd83 antibody
Anti-B-Cell Activation Protein antibody
Anti-Cell Surface Protein Hb15 antibody
Anti-Cd Antigen Cd83 antibody
Anti-CD83 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance