| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD81 (106-211) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Cd81 |
| Gene ID: |
12520 |
| Uniprot ID: |
CD81_MOUSE |
| Immunogen Region: |
106-211 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 106-211 of mouse CD81 (NP_598416.1). |
| Immunogen Sequence: |
VAAGIWGFVNKDQIAKDVKQ FYDQALQQAVMDDDANNAKA VVKTFHETLNCCGSNALTTL TTTILRNSLCPSGGNILTPL LQQDCHQKIDELFSGKLYLI GIAAIV |
| Tissue Specificity | Expressed in oocytes (at protein level). Highly expressed in granulosa cells. Expressed in skeletal muscle mainly in endothelial cells of endomysial capillaries, in satellite cells and myoblasts (at protein level). Expressed in hepatocytes (at protein level). |
| Post Translational Modifications | Not glycosylated. Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts. |
| Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells. Upon initial encounter with a microbial pathogen, enables the assembly of CD19-CR2 and B cell receptor complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and humoral immune response. In T cells, associates with CD4 or CD8 coreceptors and defines the maturation state of antigen-induced synapses with B cells. Facilitates localization of CD3 in these immune synapses, required for costimulation and sustained activation of T cells, preferentially triggering T helper type 2 immune response. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. In myoblasts, associates with another tetraspanin CD9 in complex with PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Positively regulates sperm-egg fusion and may be involved in the acrosome reaction. Regulates protein trafficking in intracellular compartments. In T cells, associates with dNTPase SAMHD1 and defines its subcellular location, enabling its degradation by the proteasome and thereby controlling intracellular dNTP levels. Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung. (Microbial infection) Specifically required for Plasmodium yoelii infectivity of hepatocytes, controlling sporozoite entry in hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms. |
| Protein Name | Cd81 Antigen26 Kda Cell Surface Protein Tapa-1Target Of The Antiproliferative Antibody 1Cd Antigen Cd81 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-977606 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneAssociates With Cldn1 And The Cldn1-Cd81 Complex Localizes To The Basolateral Cell Membrane |
| Alternative Antibody Names | Anti-Cd81 Antigen antibodyAnti-26 Kda Cell Surface Protein Tapa-1 antibodyAnti-Target Of The Antiproliferative Antibody 1 antibodyAnti-Cd Antigen Cd81 antibodyAnti-Cd81 antibodyAnti-Tapa1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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