| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-T-lymphocyte activation antigen CD80 (100-160 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-200 ELISA 1:10000-20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD80 |
| Gene ID: | 941 |
| Uniprot ID: | CD80_HUMAN |
| Immunogen Region: | 100-160 aa |
| Specificity: | The antibody detects endogenous CD80 |
| Immunogen: | Synthetic peptide from the human protein at the amino acid range 100-160 |
| Post Translational Modifications | Palmitoylated by ZDHHC20.palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization. |
| Function | Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
| Protein Name | T-Lymphocyte Activation Antigen Cd80 Activation B7-1 Antigen Bb1 Ctla-4 Counter-Receptor B7.1 B7 Cd Antigen Cd80 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-389356 Reactome: R-HSA-389357 Reactome: R-HSA-389359 Reactome: R-HSA-389513 Reactome: R-HSA-6783783 Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Lymphocyte Activation Antigen Cd80 antibody Anti-Activation B7-1 Antigen antibody Anti-Bb1 antibody Anti-Ctla-4 Counter-Receptor B7.1 antibody Anti-B7 antibody Anti-Cd Antigen Cd80 antibody Anti-CD80 antibody Anti-CD28LG antibody Anti-CD28LG1 antibody Anti-LAB7 antibody |
Information sourced from Uniprot.org