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Anti-CD80 antibody (1-100) (STJ118479)

Immunohistochemistry analysis of CD80 in paraffin-embedded human spleen using CD80 Rabbit polyclonal antibody (STJ118479) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
WWestern blot analysis of various lysates, using CD80 Rabbit polyclonal antibody (STJ118479) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Western blot analysis of lysates from Rat lung, using CD80 Rabbit polyclonal antibody (STJ118479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CD80 antibody (1-100) (STJ118479)

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STJ118479

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-CD80 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CD80
Gene ID:	941
Uniprot ID:	CD80_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD80 (NP_005182.1).
Immunogen Sequence:	MGHTRRQGTSPSKCPYLNFF QLLVLAGLSHFCSGVIHVTK EVKEVATLSCGHNVSVEELA QTRIYWQKEKKMVLTMMSGD MNIWPEYKNRTIFDITNNLS

Tissue Specificity	Expressed on activated B-cells, macrophages and dendritic cells.
Function	Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Protein Name	T-Lymphocyte Activation Antigen Cd80 Activation B7-1 Antigen Bb1 Ctla-4 Counter-Receptor B7.1 B7 Cd Antigen Cd80
Database Links	Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-389356 Reactome: R-HSA-389357 Reactome: R-HSA-389359 Reactome: R-HSA-389513 Reactome: R-HSA-6783783 Reactome: R-HSA-6811558
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative Antibody Names	Anti-T-Lymphocyte Activation Antigen Cd80 antibody Anti-Activation B7-1 Antigen antibody Anti-Bb1 antibody Anti-Ctla-4 Counter-Receptor B7.1 antibody Anti-B7 antibody Anti-Cd Antigen Cd80 antibody Anti-CD80 antibody Anti-CD28LG antibody Anti-CD28LG1 antibody Anti-LAB7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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