| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CD8 beta (70-120) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:500-1:5, 000 ELISA: 1:50, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | CD8B |
| Gene ID: | 926 |
| Uniprot ID: | CD8B_HUMAN |
| Immunogen Region: | 70-120 |
| Immunogen: | Synthetic peptide taken within amino acid region 70-120 on human T-cell surface glycoprotein CD8 Beta chain. |
| Post Translational Modifications | Phosphorylated as a consequence of T-cell activation. Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Beta Chain Cd Antigen Cd8b |
| Database Links | Reactome: R-HSA-182218 Reactome: R-HSA-198933 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Requires The Partner Cd8a For Efficient Cell Surface Expression The Heterodimer Cd8a/Cd8b Localizes To Lipid Rafts Due To Cd8b Cytoplasmic Tail Palmitoylation Isoform 2: Cell Membrane Isoform 3: Secreted Isoform 4: Cell Membrane Isoform 5: Cell Membrane Isoform 6: Secreted Isoform 7: Secreted Isoform 8: Secreted |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd8 Beta Chain antibody Anti-Cd Antigen Cd8b antibody Anti-CD8B antibody Anti-CD8B1 antibody |
Information sourced from Uniprot.org