Host: | Mouse |
Applications: | IHC/IF |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Mouse monoclonal antibody anti-CD74 is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
Clonality: | Monoclonal |
Clone ID: | SM4976 |
Conjugation: | Unconjugated |
Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Dilution Range: | IHC/IF (H) 1:200-1:500 |
Storage Instruction: | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Gene Symbol: | CD74 |
Gene ID: | 972 |
Uniprot ID: | HG2A_HUMAN |
Immunogen: | Synthesized peptide derived from human CD74 |
Post Translational Modifications | O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate. |
Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform. |
Protein Name | Hla Class Ii Histocompatibility Antigen Gamma Chain Hla-Dr Antigens-Associated Invariant Chain Ia Antigen-Associated Invariant Chain Ii Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide Clip |
Database Links | Reactome: R-HSA-202733 Reactome: R-HSA-2132295 |
Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein Endoplasmic Reticulum Membrane Golgi Apparatus Trans-Golgi Network Endosome Lysosome Secreted Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway It Can Either Undergo Proteolysis Or Reach The Cell Membrane Isoform P41: Late Endosome |
Alternative Antibody Names | Anti-Hla Class Ii Histocompatibility Antigen Gamma Chain antibody Anti-Hla-Dr Antigens-Associated Invariant Chain antibody Anti-Ia Antigen-Associated Invariant Chain antibody Anti-Ii antibody Anti-Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide antibody Anti-Clip antibody Anti-CD74 antibody Anti-DHLAG antibody |
Information sourced from Uniprot.org