• Immunofluorescence analysis of Raji cells using CD70 Rabbit polyclonal antibody (STJ23009) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells using CD70 Rabbit polyclonal antibody (STJ23009) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CD70 in paraffin-embedded human spleen using CD70 Rabbit polyclonal antibody (STJ23009) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat spleen using CD70 Rabbit polyclonal antibody (STJ23009) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.

Anti-CD70 antibody (47-126) (STJ23009)

SKU:
STJ23009

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD70
Gene ID: 970
Uniprot ID: CD70_HUMAN
Immunogen Region: 47-126
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 47-126 of human CD70 (NP_001243.1).
Immunogen Sequence: QLPLESLGWDVAELQLNHTG PQQDPRLYWQGGPALGRSFL HGPELDKGQLRIHRDGIYMV HIQVTLAICSSTTASRHHPT
Post Translational Modifications N-glycosylated.
Function Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.
Protein Name Cd70 Antigen
Cd27 Ligand
Cd27-L
Tumor Necrosis Factor Ligand Superfamily Member 7
Cd Antigen Cd70
Database Links Reactome: R-HSA-5669034
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Cd70 Antigen antibody
Anti-Cd27 Ligand antibody
Anti-Cd27-L antibody
Anti-Tumor Necrosis Factor Ligand Superfamily Member 7 antibody
Anti-Cd Antigen Cd70 antibody
Anti-CD70 antibody
Anti-CD27L antibody
Anti-CD27LG antibody
Anti-TNFSF7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance