• Flow cytometry:1×10^6 Raji cells were stained with CD70 Rabbit monoclonal antibody (STJ11104003, 1 ug/ml Blue line). Non-fluorescently stained Raji cells were used as blank control (red line).
  • Western blot analysis of extracts of various cell lines, using CD70 antibody (STJ11104003) at dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CD70 antibody (39-193) [S4003RM] (STJ11104003)

SKU:
STJ11104003

£129.50 - £574.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4003RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
FC:1:100-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD70
Gene ID: 970
Uniprot ID: CD70_HUMAN
Immunogen Region: 39-193
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 39-193 of human CD70 (P32970).
Immunogen Sequence: QRFAQAQQQLPLESLGWDVA ELQLNHTGPQQDPRLYWQGG PALGRSFLHGPELDKGQLRI HRDGIYMVHIQVTLAICSST TASRHHPTTLAVGICSPASR SISLLRLSFHQGCTIASQRL TPLARGDTLCTNLTGTLLPS RNTDETFFGVQWVRP
Post Translational Modifications N-glycosylated.
Function Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.
Protein Name Cd70 Antigen
Cd27 Ligand
Cd27-L
Tumor Necrosis Factor Ligand Superfamily Member 7
Cd Antigen Cd70
Database Links Reactome: R-HSA-5669034
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Cd70 Antigen antibody
Anti-Cd27 Ligand antibody
Anti-Cd27-L antibody
Anti-Tumor Necrosis Factor Ligand Superfamily Member 7 antibody
Anti-Cd Antigen Cd70 antibody
Anti-CD70 antibody
Anti-CD27L antibody
Anti-CD27LG antibody
Anti-TNFSF7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance