Anti-CD68 antibody (22-319) (STJ115250)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD68 (22-319) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD68 |
| Gene ID | 968 |
| Uniprot ID | CD68_HUMAN |
| Immunogen Region | 22-319 |
| Immunogen Sequence | NDCPHKKSATLLPSFTVTPT VTESTGTTSHRTTKSHKTTT HRTTTTGTTSHGPTTATHNP TTTSHGNVTVHPTSNSTATS QGPSTATHSPATTSHGNATV HPTSNSTATSPGFTSSAHPE PPPPSPSPSPTSKETIGDYT WTNGSQPCVHLQAQIQIRVM YTTQGGGEAWGISVLNPNKT KVQGSCEGAHPHLLLSFPYG HLSFGFMQDLQQKVVYLSYM AVEYNVSFPHAAQWTFSAQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 22-319 of human CD68 (NP_001242.2). |
Additional Info
| Tissue Specificity | Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
| Protein Name | MacrosialinGp110Cd Antigen Cd68 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Short: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Long: Endosome MembraneLysosome Membrane |
| Alternative Antibody Names | Anti-Macrosialin antibodyAnti-Gp110 antibodyAnti-Cd Antigen Cd68 antibodyAnti-CD68 antibody |
Information sourced from Uniprot.org