• Anti-CD68 antibody (22-319) (STJ115250)
  • Immunofluorescence analysis of RAW264. 7 cells using CD68 Rabbit polyclonal antibody (STJ115250) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD68 Rabbit polyclonal antibody (STJ115250) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using CD68 Rabbit polyclonal antibody (STJ115250) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CD68 Rabbit polyclonal antibody (STJ115250) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of THP-1, using CD68 antibody (STJ115250) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD68 antibody (22-319) (STJ115250)

SKU:
STJ115250

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD68 (22-319) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD68
Gene ID: 968
Uniprot ID: CD68_HUMAN
Immunogen Region: 22-319
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 22-319 of human CD68 (NP_001242.2).
Immunogen Sequence: NDCPHKKSATLLPSFTVTPT VTESTGTTSHRTTKSHKTTT HRTTTTGTTSHGPTTATHNP TTTSHGNVTVHPTSNSTATS QGPSTATHSPATTSHGNATV HPTSNSTATSPGFTSSAHPE PPPPSPSPSPTSKETIGDYT WTNGSQPCVHLQAQIQIRVM YTTQGGGEAWGISVLNPNKT KVQGSCEGAHPHLLLSFPYG HLSFGFMQDLQQKVVYLSYM AVEYNVSFPHAAQWTFSAQ
Tissue Specificity Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Post Translational Modifications N- and O-glycosylated.
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Protein Name Macrosialin
Gp110
Cd Antigen Cd68
Database Links Reactome: R-HSA-6798695
Cellular Localisation Isoform Short: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Long: Endosome Membrane
Lysosome Membrane
Alternative Antibody Names Anti-Macrosialin antibody
Anti-Gp110 antibody
Anti-Cd Antigen Cd68 antibody
Anti-CD68 antibody

Information sourced from Uniprot.org

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