| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD68 (16-200) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CD68 |
| Gene ID: |
968 |
| Uniprot ID: |
CD68_HUMAN |
| Immunogen Region: |
16-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human CD68 (NP_001242.2). |
| Immunogen Sequence: |
AAQGTGNDCPHKKSATLLPS FTVTPTVTESTGTTSHRTTK SHKTTTHRTTTTGTTSHGPT TATHNPTTTSHGNVTVHPTS NSTATSQGPSTATHSPATTS HGNATVHPTSNSTATSPGFT SSAHPEPPPPSPSPSPTSKE TIGDYTWTNGSQPCVHLQAQ IQIRVMYTTQGGGEAWGISV LNPNK |
| Tissue Specificity | Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
| Protein Name | MacrosialinGp110Cd Antigen Cd68 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Short: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Long: Endosome MembraneLysosome Membrane |
| Alternative Antibody Names | Anti-Macrosialin antibodyAnti-Gp110 antibodyAnti-Cd Antigen Cd68 antibodyAnti-CD68 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CD68 Rabbit polyclonal antibody knockout (KO) U-87MG cells, using [KO Validated] CD68 Rabbit polyclonal antibody (STJ11104713) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177357/369051/STJ11104713_1__51226.1713134535.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD68 Rabbit polyclonal antibody knockout (KO) U-87MG cells, using [KO Validated] CD68 Rabbit polyclonal antibody (STJ11104713) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
