Anti-CD5L antibody [SAA1515] (STJA0019943)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
General Information
| Short Description | Mouse monoclonal Cd5 antigen-Like antibody for use in ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO) MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA1515 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Protein A/G purified from cell culture supernatant |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks For longer term store at-20°C for up to 12 months |
Target Information
| Gene Symbol | CD5L |
| Gene ID | 922 |
| Uniprot ID | CD5L_HUMAN |
Additional Info
| Post Translational Modifications | Not N-glycosylated. Probably not O-glycosylated. |
| Function | Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis. Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy. |
| Protein Name | Cd5 Antigen-LikeApoptosis Inhibitor Expressed By MacrophagesHaimCt-2Igm-Associated PeptideSp-Alpha |
| Database Links | |
| Cellular Localisation | SecretedCytoplasmSecreted By Macrophages And Circulates In The BloodTransported In The Cytoplasm Via Cd36-Mediated Endocytosis |
| Alternative Antibody Names | Anti-Cd5 Antigen-Like antibodyAnti-Apoptosis Inhibitor Expressed By Macrophages antibodyAnti-Haim antibodyAnti-Ct-2 antibodyAnti-Igm-Associated Peptide antibodyAnti-Sp-Alpha antibodyAnti-CD5L antibodyAnti-API6 antibodyAnti-UNQ203 antibodyAnti-PRO229 antibody |
Information sourced from Uniprot.org