• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD52 antibody (STJ11101181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD52 antibody (STJ11101181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse bone marrow, using CD52 Rabbit polyclonal antibody (STJ11101181) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD52 antibody (25-41) (STJ11101181)

SKU:
STJ11101181

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD52 (25-41) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD52
Gene ID: 1043
Uniprot ID: CD52_HUMAN
Immunogen Region: 25-41
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 25-41 of human CD52 (NP_001794.2).
Immunogen Sequence: GQNDTSQTSSPSASSNI
Function May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Protein Name Campath-1 Antigen
Cdw52
Cambridge Pathology 1 Antigen
Epididymal Secretory Protein E5
Human Epididymis-Specific Protein 5
He5
Cd Antigen Cd52
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Campath-1 Antigen antibody
Anti-Cdw52 antibody
Anti-Cambridge Pathology 1 Antigen antibody
Anti-Epididymal Secretory Protein E5 antibody
Anti-Human Epididymis-Specific Protein 5 antibody
Anti-He5 antibody
Anti-Cd Antigen Cd52 antibody
Anti-CD52 antibody
Anti-CDW52 antibody
Anti-HE5 antibody

Information sourced from Uniprot.org

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