Anti-CD50 antibody [101-1D2] (STJ16101111)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenA BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1.
General Information
| Short Description | Mouse monoclonal anti-CD50 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 101-1D2 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 ยตg/mL, 500 ยตg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (1-2 ยตg/million cells in 0, 1 ml). Immunofluorescence (1-2 ยตg/ml). Immunohistology (1-2 ยตg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed b |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8ยฐC upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1. |
Additional Info
| Background | 101-1D2 reacts with the cellular adhesion molecule CD50 (ICAM-3) , a single chain polypeptide with a MW of 12 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PO-anchored. 101-1D2 was provisionally clustered as CDw50 at the IV and confirmed as CD50 at the V international workshop on human leucocyte differentiation antigens. |
Information sourced from Uniprot.org