| Short Description : | Mouse monoclonal anti-CD50 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | FC/IF/IHC |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 101-1D2 |
| Isotype: | IgG1k |
| Conjugation: | Unconjugated |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification: | Affinity purified from tissue culture. |
| Dilution Range: | Flow cytometry (1-2 µg/million cells in 0, 1 ml). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed b |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1. |
| Background | 101-1D2 reacts with the cellular adhesion molecule CD50 (ICAM-3) , a single chain polypeptide with a MW of 12 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PO-anchored. 101-1D2 was provisionally clustered as CDw50 at the IV and confirmed as CD50 at the V international workshop on human leucocyte differentiation antigens. |
Information sourced from Uniprot.org
