Host: | Mouse |
Applications: | IHC/WB |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Mouse monoclonal antibody anti-T-cell surface glycoprotein CD5 (200-300 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
Clonality: | Monoclonal |
Clone ID: | ABT-CD5 |
Conjugation: | Unconjugated |
Isotype: | IgG2ak |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Dilution Range: | IHC-P 1:100-500 WB 1:200-1000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CD5 |
Gene ID: | 921 |
Uniprot ID: | CD5_HUMAN |
Immunogen Region: | 200-300 aa |
Specificity: | This antibody detects endogenous levels of human CD5. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section |
Immunogen: | Synthesized peptide derived from the human CD5 at the amino acid range 200-300 |
Function | Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production. |
Protein Name | T-Cell Surface Glycoprotein Cd5 Lymphocyte Antigen T1/Leu-1 Cd Antigen Cd5 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd5 antibody Anti-Lymphocyte Antigen T1/Leu-1 antibody Anti-Cd Antigen Cd5 antibody Anti-CD5 antibody Anti-LEU1 antibody |
Information sourced from Uniprot.org