• Western blot analysis of various lysates, using CD48 antibody (STJ11103550) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Daudi cells (right) were surface-stained with CD48 rabbit monoclonal antibody (STJ11103550, 10 Mu g/mL orange line) or ABflo® 647 rabbit IgG isotype control (10 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Anti-CD48 antibody (27-220) [S0MR] (STJ11103550)

SKU:
STJ11103550

£146.50 - £639.50
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Host: Rabbit
Applications: WB/FC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD48 (27-220) is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD48
Gene ID: 962
Uniprot ID: CD48_HUMAN
Immunogen Region: 27-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-220 of human CD48 (NP_001769.2).
Immunogen Sequence: QGHLVHMTVVSGSNVTLNIS ESLPENYKQLTWFYTFDQKI VEWDSRKSKYFESKFKGRVR LDPQSGALYISKVQKEDNST YIMRVLKKTGNEQEWKIKLQ VLDPVPKPVIKIEKIEDMDD NCYLKLSCVIPGESVNYTWY GDKRPFPKELQNSVLETTLM PHNYSRCYTCQVSNSVSSKN GTVCLSPPCTLARS
Tissue Specificity Widely expressed on all hematopoietic cells.
Function Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation. Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn, promotes LCK phosphorylation and subsequent activation. Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells.
Protein Name Cd48 Antigen
B-Lymphocyte Activation Marker Blast-1
Bcm1 Surface Antigen
Leukocyte Antigen Mem-102
Slam Family Member 2
Slamf2
Signaling Lymphocytic Activation Molecule 2
Tct.1
Cd Antigen Cd48
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Alternative Antibody Names Anti-Cd48 Antigen antibody
Anti-B-Lymphocyte Activation Marker Blast-1 antibody
Anti-Bcm1 Surface Antigen antibody
Anti-Leukocyte Antigen Mem-102 antibody
Anti-Slam Family Member 2 antibody
Anti-Slamf2 antibody
Anti-Signaling Lymphocytic Activation Molecule 2 antibody
Anti-Tct.1 antibody
Anti-Cd Antigen Cd48 antibody
Anti-CD48 antibody
Anti-BCM1 antibody
Anti-BLAST1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance