Anti-CD47 Antibody antibody (ECD) [M054] (STJA0041103)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone (M054) was generated from a recombinant protein that included the extracellular region of human CD47 protein.
General Information
| Short Description | Mouse monoclonal anti-CD47 antibody (ECD) for use in ELISA, WB-Nat, ICC and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB-Nat/ICC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M054 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:500ICC 1:200IP 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | CD47 |
| Gene ID | 961 |
| Uniprot ID | CD47_HUMAN |
| Immunogen | Clone (M054) was generated from a recombinant protein that included the extracellular region of human CD47 protein. |
| Immunogen Region | ECD |
| Immunogen Sequence | Human |
| Specificity | Clone M054 mouse monoclonal antibody detects native human CD47, as well as aldehyde-fixed CD47. The antibody works for staining in immunocytochemistry, capture and detection in ELISA, native immunoprecipitation and western blot. The antibody detects |
Additional Info
| Tissue Specificity | Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. Macrophages. |
| Function | Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering. Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging. In response to THBS1, negatively modulates wound healing. Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. |
| Protein Name | Leukocyte Surface Antigen Cd47Antigenic Surface Determinant Protein Oa3Integrin-Associated ProteinIapProtein Mer6Cd Antigen Cd47 |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-391160Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Leukocyte Surface Antigen Cd47 antibodyAnti-Antigenic Surface Determinant Protein Oa3 antibodyAnti-Integrin-Associated Protein antibodyAnti-Iap antibodyAnti-Protein Mer6 antibodyAnti-Cd Antigen Cd47 antibodyAnti-CD47 antibodyAnti-MER6 antibody |
Information sourced from Uniprot.org