| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC/FC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CD4 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 30kDa band observed in Human Spleen and Bone Marrow lysates (calculated MW of 31.3kDa according to NP_001181943.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CD4 |
| Gene ID: | 920 |
| Uniprot ID: | CD4_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000607.1; NP_001181943.1 |
| Specificity: | This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1). |
| Immunogen Sequence: | KNKEVSVKRVTQDPK |
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation at Ser-433 plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7. |
| Protein Name | T-Cell Surface Glycoprotein Cd4 T-Cell Surface Antigen T4/Leu-3 Cd Antigen Cd4 |
| Database Links | Reactome: R-HSA-1462054 Reactome: R-HSA-167590 Reactome: R-HSA-173107 Reactome: R-HSA-180534 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-389948 Reactome: R-HSA-449836 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Localizes To Lipid Rafts Removed From Plasma Membrane By Hiv-1 Nef Protein That Increases Clathrin-Dependent Endocytosis Of This Antigen To Target It To Lysosomal Degradation Cell Surface Expression Is Also Down-Modulated By Hiv-1 Envelope Polyprotein Gp160 That Interacts With And Sequesters Cd4 In The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibody Anti-T-Cell Surface Antigen T4/Leu-3 antibody Anti-Cd Antigen Cd4 antibody Anti-CD4 antibody |
Information sourced from Uniprot.org