| Host: |
Rat |
| Applications: |
FC |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-T-cell surface glycoprotein CD4 is suitable for use in Flow Cytometry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
GK1.5 |
| Conjugation: |
AF488 |
| Isotype: |
IgG2bk |
| Formulation: |
PBS with 0.05% Proclin300, 1% BSA |
| Storage Instruction: |
Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
| Tissue Specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
| Protein Name | T-Cell Surface Glycoprotein Cd4T-Cell Differentiation Antigen L3t4T-Cell Surface Antigen T4/Leu-3Cd Antigen Cd4 |
| Database Links | Reactome: R-MMU-1462054Reactome: -MMU-202424Reactome: -MMU-202427Reactome: -MMU-202430Reactome: -MMU-202433Reactome: -MMU-389948Reactome: -MMU-449836Reactome: -MMU-8856825Reactome: -MMU-8856828 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibodyAnti-T-Cell Differentiation Antigen L3t4 antibodyAnti-T-Cell Surface Antigen T4/Leu-3 antibodyAnti-Cd Antigen Cd4 antibodyAnti-Cd4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Mouse splenocytes were stained with Anti-CD4 antibody (STJA0000535) [Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/145388/292050/STJA0000535_1__48202.1631205878.jpg?c=1 "Mouse splenocytes were stained with Anti-CD4 antibody (STJA0000535) [Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.")
