| Host: | Rabbit |
| Applications: | WB/IHC-P/ICC/IF/IP/FC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-CD3G for use in WB, IHC-P, ICC, IF, IP and FC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R07-1Q8 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification: | Affinity Chromatography |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | CD3G |
| Gene ID: | 917 |
| Uniprot ID: | CD3G_HUMAN |
| Immunogen: | A synthesized peptide derived from human CD3G |
| Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC.leading to the TCR complex down-regulation. Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Gamma ChainT-Cell Receptor T3 Gamma ChainCd Antigen Cd3g |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-2029481Reactome: R-HSA-2029482Reactome: R-HSA-2029485Reactome: R-HSA-389948Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9664323Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Gamma Chain antibodyAnti-T-Cell Receptor T3 Gamma Chain antibodyAnti-Cd Antigen Cd3g antibodyAnti-CD3G antibodyAnti-T3G antibody |
Information sourced from Uniprot.org
