| Host: |
Rabbit |
| Applications: |
IHC-P/ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
IHC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
Cd3e |
| Gene ID: |
12501 |
| Uniprot ID: |
CD3E_MOUSE |
| Immunogen Region: |
23-108 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 23-108 of mouse CD3E (NP_031674.1). |
| Immunogen Sequence: |
DAENIEYKVSISGTSVELTC PLDSDENLKWEKNGQELPQK HDKHLVLQDFSEVEDSGYYV CYTPASNKNTYLYLKARVCE YCVEVD |
| Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Epsilon ChainT-Cell Surface Antigen T3/Leu-4 Epsilon ChainCd Antigen Cd3e |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-202424Reactome: -MMU-202427Reactome: -MMU-202430Reactome: -MMU-202433Reactome: -MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibodyAnti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibodyAnti-Cd Antigen Cd3e antibodyAnti-Cd3e antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
