• Confocal immunofluorescent analysis of Jurkat cells labeling CD3 (ABT295) Mouse monoclonal antibody followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green). Counterstained with DAPI (blue). Negative control: PBS was used to instead of primary antibody, followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green).
  • Whole cell lysates of Jurkat were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD3 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 23 kDa
  • Human appendix tissue was stained with Anti-CD3 (ABT295) Antibody
  • Human spleen tissue was stained with Anti-CD3 (ABT295) Antibody
  • Human tonsil tissue was stained with Anti-CD3 (ABT295) Antibody
  • Human tonsil tissue was stained with Anti-CD3 (ABT295) Antibody

Anti-CD3E antibody (1-100 aa) [ABT295] (STJ197119)

SKU:
STJ197119

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/ICC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-T-cell surface glycoprotein CD3 epsilon chain (1-100 aa) is suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT295
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3E
Gene ID: 916
Uniprot ID: CD3E_HUMAN
Immunogen Region: 1-100 aa
Specificity: The antibody can specifically recognize human CD3e protein, and shows no reaction with CD3d or CD3g. In western blotting of Jurkat cell lysate, the antibody can label a 23KDa band corresponding to CD
Immunogen: Synthesized peptide derived from the human CD3, CD3E at the amino acid range 1-100
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Protein Name T-Cell Surface Glycoprotein Cd3 Epsilon Chain
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain
Cd Antigen Cd3e
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibody
Anti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibody
Anti-Cd Antigen Cd3e antibody
Anti-CD3E antibody
Anti-T3E antibody

Information sourced from Uniprot.org

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