• Western blot analysis of extracts of Jurkat cells, using CD3D rabbit monoclonal antibody (STJ11102545) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were surface-stained with CD3D Rabbit monoclonal antibody (STJ11102545, 2. 5 Mu g/mL orange line) or ABflo® 488 IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).
  • Immunofluorescence analysis of rat spleen using CD3D rabbit monoclonal antibody (STJ11102545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of paraffin-embedded Human spleen tissue using CD3D Rabbit monoclonal antibody (STJ11102545, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of mouse spleen using CD3D rabbit monoclonal antibody (STJ11102545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Jurkat cells, using CD3D Rabbit monoclonal antibody (STJ11102545) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Flow cytometry: Jurkat cells were stained with rabbit IgG isotype control ( (10 ug/ml, blue line) or CD3D rabbit monoclonal antibody (STJ11102545, 2. 5 ug/ml orange line) , followed by goat anti-rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).
  • Flow cytometry: Raji cells were stained with rabbit IgG isotype control ( (10 ug/ml, blue line) or CD3D rabbit monoclonal antibody (STJ11102545, 2. 5 ug/ml orange line) , followed by goat anti-rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line).

Anti-CD3D antibody (72-171) [S5MR] (STJ11102545)

SKU:
STJ11102545

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD3D (72-171) is suitable for use in Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
FC:1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3D
Gene ID: 915
Uniprot ID: CD3D_HUMAN
Immunogen Region: 72-171
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 72-171 of human CD3D (P04234).
Immunogen Sequence: RCNGTDIYKDKESTVQVHYR MCQSCVELDPATVAGIIVTD VIATLLLALGVFCFAGHETG RLSGAADTQALLRNDQVYQP LRDRDDAQYSHLGGNWARNK
Tissue Specificity CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells.
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
Protein Name T-Cell Surface Glycoprotein Cd3 Delta Chain
T-Cell Receptor T3 Delta Chain
Cd Antigen Cd3d
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Delta Chain antibody
Anti-T-Cell Receptor T3 Delta Chain antibody
Anti-Cd Antigen Cd3d antibody
Anti-CD3D antibody
Anti-T3D antibody

Information sourced from Uniprot.org

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