![Anti-CD33 antibody [WM53] {PE} (STJ16101385)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/151894/346979/STJ16101385_1__94140.1681999215.jpg?c=1 "Anti-CD33 antibody [WM53] {PE} (STJ16101385)")
![Anti-CD33 antibody [WM53] {PE} (STJ16101385)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/151894/346979/STJ16101385_1__94140.1681999215.jpg?c=1 "Anti-CD33 antibody [WM53] {PE} (STJ16101385)")
| Host: | Mouse |
| Applications: | FC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CD33 is suitable for use in Flow Cytometry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | WM53 |
| Conjugation: | PE |
| Isotype: | IgG1 |
| Formulation: | PBS with 0.09% sodium azide and 1% BSA, 5%sucrose |
| Storage Instruction: | Store at 2-8°C upon receipt. |
| Gene Symbol: | CD33 |
| Gene ID: | 945 |
| Uniprot ID: | CD33_HUMAN |
| Immunogen: | Human AML cells |
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33 Sialic Acid-Binding Ig-Like Lectin 3 Siglec-3 Gp67 Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell Membrane Single-Pass Type I Membrane Protein Isoform Cd33m: Peroxisome Cd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Antibody Names | Anti-Myeloid Cell Surface Antigen Cd33 antibody Anti-Sialic Acid-Binding Ig-Like Lectin 3 antibody Anti-Siglec-3 antibody Anti-Gp67 antibody Anti-Cd Antigen Cd33 antibody Anti-CD33 antibody Anti-SIGLEC3 antibody |
Information sourced from Uniprot.org