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![Anti-CD33 antibody [WM53] {FITC} (STJ16100132)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/148850/346528/STJ16100132_1__44537.1681998573.jpg?c=1 "Anti-CD33 antibody [WM53] {FITC} (STJ16100132)")
Anti-CD33 antibody [WM53] {FITC} (STJ16100132)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationFITC
IsotypeIgG1
ImmunogenHuman AML cells
General Information
| Short Description | Mouse monoclonal anti-CD33 for use in FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | WM53 |
| Isotype | IgG1 |
| Conjugation | FITC |
| Concentration | 0.1 mg/mL |
| Dilution Range | FC 5 ยตL 0.5 ยตg/test. |
| Formulation | PBS with 0.09% sodium azide and 1% BSA |
| Storage Instruction | Store at 2-8ยฐC for up to 1-year, upon receipt. |
Target Information
| Gene Symbol | CD33 |
| Gene ID | 945 |
| Uniprot ID | CD33_HUMAN |
| Immunogen | Human AML cells |
Additional Info
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Antibody Names | Anti-Myeloid Cell Surface Antigen Cd33 antibodyAnti-Sialic Acid-Binding Ig-Like Lectin 3 antibodyAnti-Siglec-3 antibodyAnti-Gp67 antibodyAnti-Cd Antigen Cd33 antibodyAnti-CD33 antibodyAnti-SIGLEC3 antibody |
Information sourced from Uniprot.org