• Anti-CD33 antibody [WM53] {FITC} (STJ16100132)

Anti-CD33 antibody [WM53] {FITC} (STJ16100132)

SKU:
STJ16100132-01

£620.50
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Host: Mouse
Applications: FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD33 is suitable for use in Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: WM53
Conjugation: FITC
Isotype: IgG1
Formulation: PBS with 0.09% sodium azide and 1% BSA
Concentration: 0.1 mg/mL
Dilution Range: FC 5 µL 0.5 µg/test.
Storage Instruction: Store at 2-8°C upon receipt.
Gene Symbol: CD33
Gene ID: 945
Uniprot ID: CD33_HUMAN
Immunogen: Human AML cells
Tissue Specificity Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.
Post Translational Modifications Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.
Function Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Protein Name Myeloid Cell Surface Antigen Cd33
Sialic Acid-Binding Ig-Like Lectin 3
Siglec-3
Gp67
Cd Antigen Cd33
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-6798695
Cellular Localisation Isoform Cd33m: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Cd33m: Peroxisome
Cd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes
Alternative Antibody Names Anti-Myeloid Cell Surface Antigen Cd33 antibody
Anti-Sialic Acid-Binding Ig-Like Lectin 3 antibody
Anti-Siglec-3 antibody
Anti-Gp67 antibody
Anti-Cd Antigen Cd33 antibody
Anti-CD33 antibody
Anti-SIGLEC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance