• Western blot analysis of various lysates using CD324/E-Cadherin Rabbit polyclonal antibody (STJ11105515) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : HeLa. Exposure time: 5s.

Anti-CD324/E-Cadherin antibody (157-369) (STJ11105515)

SKU:
STJ11105515

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD324/E-Cadherin (157-369) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cdh1
Gene ID: 12550
Uniprot ID: CADH1_MOUSE
Immunogen Region: 157-369
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 157-369 of mouse CD324/E-Cadherin (NP_033994.1).
Immunogen Sequence: WVIPPISCPENEKGEFPKNL VQIKSNRDKETKVFYSITGQ GADKPPVGVFIIERETGWLK VTQPLDREAIAKYILYSHAV SSNGEAVEDPMEIVITVTDQ NDNRPEFTQEVFEGSVAEGA VPGTSVMKVSATDADDDVNT YNAAIAYTIVSQDPELPHKN MFTVNRDTGVISVLTSGLDR ESYPTYTLVVQAADLQGEGL STTAKAVITVKD
Tissue Specificity Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Non-neural epithelial tissues.
Post Translational Modifications During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA).mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.
Protein Name Cadherin-1
Arc-1
Epithelial Cadherin
E-Cadherin
Uvomorulin
Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3
Database Links Reactome: R-MMU-1474228
Reactome: -MMU-216083
Reactome: -MMU-351906
Reactome: -MMU-418990
Reactome: -MMU-5626467
Cellular Localisation Cell Junction
Adherens Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Endosome
Golgi Apparatus
Trans-Golgi Network
Colocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Anchored To Actin Microfilaments Through Association With Alpha-
Beta- And Gamma-Catenin
Sequential Proteolysis Induced By Apoptosis Or Calcium Influx
Results In Translocation From Sites Of Cell-Cell Contact To The Cytoplasm
Colocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane
Alternative Antibody Names Anti-Cadherin-1 antibody
Anti-Arc-1 antibody
Anti-Epithelial Cadherin antibody
Anti-E-Cadherin antibody
Anti-Uvomorulin antibody
Anti-Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody
Anti-Cdh1 antibody

Information sourced from Uniprot.org

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