Anti-CD32 antibody [SRM13515] (STJ11108245)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal CD32 antibody for use in IHC-P, FC and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/FC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13515 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P, 1:50-1:200FC, 1:500-1:1000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3 |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Fcgr2 |
| Gene ID | 14130 |
| Uniprot ID | FCGR2_MOUSE |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Immunogen Sequence | Mouse |
Additional Info
| Post Translational Modifications | Glycosylated. When coaggregated to BCR, isoform IIB1 and isoform IIB1' become tyrosine phosphorylated and bind to the SH2 domains of the protein tyrosine phosphatase PTPC1. Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN and SYK. |
| Function | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively. |
| Protein Name | Low Affinity Immunoglobulin Gamma Fc Region Receptor IiFc Gamma Receptor IibFc-Gamma RiiFc-Gamma-RiibFcriiIgg Fc Receptor Ii BetaLymphocyte Antigen 17Ly-17Cd Antigen Cd32 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-2029481Reactome: -MMU-2029482Reactome: -MMU-2029485Reactome: -MMU-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Iib1: CytoplasmCytoskeletonBinds The Cytoskeleton And Is Not Localized In Endocytotic PitsIsoform Iib3: SecretedReleased As A Soluble Molecule |
| Alternative Antibody Names | Anti-Low Affinity Immunoglobulin Gamma Fc Region Receptor Ii antibodyAnti-Fc Gamma Receptor Iib antibodyAnti-Fc-Gamma Rii antibodyAnti-Fc-Gamma-Riib antibodyAnti-Fcrii antibodyAnti-Igg Fc Receptor Ii Beta antibodyAnti-Lymphocyte Antigen 17 antibodyAnti-Ly-17 antibodyAnti-Cd Antigen Cd32 antibodyAnti-Fcgr2 antibodyAnti-Fcgr2b antibodyAnti-Ly-17 antibody |
Information sourced from Uniprot.org