Anti-CD31/PECAM1 antibody {PerCP-Cy5.5} (STJ11109084)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationPerCP-Cy5.5
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal CD31/PECAM1 antibody for use in FC in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | PerCP-Cy5.5 |
| Purification | Affinity purification |
| Dilution Range | FC, 5 Mu l per 10^6 cells in 100 Mu l volume |
| Formulation | PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3. |
| Storage Instruction | Store at 4°C for up to 1 year from the date of receipt. Do not freeze. |
Target Information
| Gene Symbol | Pecam1 |
| Gene ID | 18613 |
| Uniprot ID | PECA1_MOUSE |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Isoform 1: Expressed in lung and platelets (at protein level). Isoform 3: Expressed in lung and platelets (at protein level). |
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues by src kinases after cellular activation. Upon activation, phosphorylated on Ser-718 which probably initiates the dissociation of the membrane-interaction segment (residues 698-718) from the cell membrane allowing the sequential phosphorylation of Tyr-702 and Tyr-679. Constitutively phosphorylated on Ser-723 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. |
| Protein Name | Platelet Endothelial Cell Adhesion MoleculePecam-1Cd Antigen Cd31 |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-202733Reactome: -MMU-210990Reactome: -MMU-216083Reactome: -MMU-432142Reactome: -MMU-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftCell JunctionLocalizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial CellsCell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation |
| Alternative Antibody Names | Anti-Platelet Endothelial Cell Adhesion Molecule antibodyAnti-Pecam-1 antibodyAnti-Cd Antigen Cd31 antibodyAnti-Pecam1 antibodyAnti-Pecam antibodyAnti-Pecam-1 antibody |
Information sourced from Uniprot.org