• Immunohistochemistry analysis of CD31. Sample: Rat skeletal muscle, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:200, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: mouse lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: mouse heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: mouse lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: Rat lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: Rat heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of CD31. Sample: Rat lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.

Anti-CD31 antibody [SM5172] (STJA0005172)

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STJA0005172

£170.50 - £233.50
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Host: Mouse
Applications: IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD31 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5172
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC/IF (M, R) 1:200-1:400/1:200-1:1000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Pecam1
Gene ID: 18613
Uniprot ID: PECA1_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse CD31
Post Translational Modifications Phosphorylated on Ser and Tyr residues by src kinases after cellular activation. Upon activation, phosphorylated on Ser-718 which probably initiates the dissociation of the membrane-interaction segment (residues 698-718) from the cell membrane allowing the sequential phosphorylation of Tyr-702 and Tyr-679. Constitutively phosphorylated on Ser-723 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
Protein Name Platelet Endothelial Cell Adhesion Molecule
Pecam-1
Cd Antigen Cd31
Database Links Reactome: R-MMU-114608
Reactome: -MMU-202733
Reactome: -MMU-210990
Reactome: -MMU-216083
Reactome: -MMU-432142
Reactome: -MMU-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Cell Junction
Localizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial Cells
Cell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation
Alternative Antibody Names Anti-Platelet Endothelial Cell Adhesion Molecule antibody
Anti-Pecam-1 antibody
Anti-Cd Antigen Cd31 antibody
Anti-Pecam1 antibody
Anti-Pecam antibody
Anti-Pecam-1 antibody

Information sourced from Uniprot.org

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