• Western blot analysis of extracts of A-549 cells, using CD30 antibody (STJ11103680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of rat spleen using CD30 Rabbit monoclonal antibody (STJ11103680) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using CD30 rabbit monoclonal antibody (STJ11103680) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using CD30 Rabbit monoclonal antibody (STJ11103680) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using CD30 rabbit monoclonal antibody (STJ11103680) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of A-549 cells, using CD30 antibody (STJ11103680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CD30 antibody (496-595) [S0MR] (STJ11103680)

SKU:
STJ11103680

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD30 (496-595) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFRSF8
Gene ID: 943
Uniprot ID: TNR8_HUMAN
Immunogen Region: 496-595
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 496-595 of human CD30 (P28908).
Immunogen Sequence: SPRDLPEPRVSTEHTNNKIE KIYIMKADTVIVGTVKAELP EGRGLAGPAEPELEEELEAD HTPHYPEQETEPPLGSCSDV MLSVEEEGKEDPLPTAASGK
Tissue Specificity Isoform 2: Detected in alveolar macrophages (at protein level).
Post Translational Modifications Phosphorylated on serine and tyrosine residues (Probable). Isoform 2 is constitutively phosphorylated.
Function Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 8
Cd30l Receptor
Ki-1 Antigen
Lymphocyte Activation Antigen Cd30
Cd Antigen Cd30
Database Links Reactome: R-HSA-5669034
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 8 antibody
Anti-Cd30l Receptor antibody
Anti-Ki-1 Antigen antibody
Anti-Lymphocyte Activation Antigen Cd30 antibody
Anti-Cd Antigen Cd30 antibody
Anti-TNFRSF8 antibody
Anti-CD30 antibody
Anti-D1S166E antibody

Information sourced from Uniprot.org

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