| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CD27 (161-175) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 45kDa band observed in Human Spleen, Tonsil and Thymus lysates (calculated MW of 29.2kDa according to NP_001233.1). The observed molecular weight corresponds to the glycosylate |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CD27 |
| Gene ID: | 939 |
| Uniprot ID: | CD27_HUMAN |
| Immunogen Region: | 161-175 |
| Accession Number: | NP_001233.1 |
| Immunogen Sequence: | QTLADFRQLPARTLS |
| Post Translational Modifications | Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. |
| Protein Name | Cd27 Antigen Cd27l Receptor T-Cell Activation Antigen Cd27 T14 Tumor Necrosis Factor Receptor Superfamily Member 7 Cd Antigen Cd27 |
| Database Links | Reactome: R-HSA-5669034 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Cd27 Antigen antibody Anti-Cd27l Receptor antibody Anti-T-Cell Activation Antigen Cd27 antibody Anti-T14 antibody Anti-Tumor Necrosis Factor Receptor Superfamily Member 7 antibody Anti-Cd Antigen Cd27 antibody Anti-CD27 antibody Anti-TNFRSF7 antibody |
Information sourced from Uniprot.org