• Confocal imaging of human tonsil using CD27 Rabbit monoclonal antibody (STJ11101350, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Western blot analysis of extracts from various cell lines, using CD27 Rabbit monoclonal antibody (STJ11101350) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (1-100) [S0MR] (STJ11101350)

SKU:
STJ11101350

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD27 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD27
Gene ID: 939
Uniprot ID: CD27_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD27 (P26842).
Immunogen Sequence: MARPHPWWLCVLGTLVGLSA TPAPKSCPERHYWAQGKLCC QMCEPGTFLVKDCDQHRKAA QCDPCIPGVSFSPDHHTRPH CESCRHCNSGLLVRNCTITA
Tissue Specificity Found in most T-lymphocytes.
Post Translational Modifications Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein Name Cd27 Antigen
Cd27l Receptor
T-Cell Activation Antigen Cd27
T14
Tumor Necrosis Factor Receptor Superfamily Member 7
Cd Antigen Cd27
Database Links Reactome: R-HSA-5669034
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd27 Antigen antibody
Anti-Cd27l Receptor antibody
Anti-T-Cell Activation Antigen Cd27 antibody
Anti-T14 antibody
Anti-Tumor Necrosis Factor Receptor Superfamily Member 7 antibody
Anti-Cd Antigen Cd27 antibody
Anti-CD27 antibody
Anti-TNFRSF7 antibody

Information sourced from Uniprot.org

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