| Short Description : | Mouse monoclonal anti-CD25/IL2RA for use in ELISA, FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/FC/IF/IHC |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-CD-017 |
| Isotype: | IgG2ak |
| Conjugation: | Unconjugated |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification: | Affinity purified from tissue culture. |
| Dilution Range: | ELISA (solid phase: 0, 1-100 µg/ml; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow Cytometry (1-2 Mu g/million cells in 0, 1 ml for 30 min, at 4°C). Immunofluorescence (1-2 Mu g/ml). Immunohistology (1-2 Mu g/ml for 30 min at RT; no suitable antige |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with human PBMCs. Fusion partner: Sp2/0. |
| Background | EBS-CD-017 reacts with CD25 (55 kDa) which associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin s disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hairy cell leukemia. Increased levels of soluble CD25 are observed in leukemias/lymphomas and inflammatory/autoimmune diseases. CD25 alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. EBS-CD-017 recognizes B epitope, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25. CD25 antibodies have been used successfully as a carrier for cytotoxic drugs enabling specific delivery to IL-2RA displaying target cells. |
Information sourced from Uniprot.org