• Flow cytometric analysis of Jurkat cells using CD247 monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded human Thymus tissues with AEC staining using CD247 monoclonal antibody.
  • Immunofluorescence analysis of K562 cells using CD247 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using CD247 monoclonal antibody against CD247-hIgGFc transfected HEK293 cell lysate.

Anti-CD247 antibody [4B10] (STJ97916)

SKU:
STJ97916

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-T-cell surface glycoprotein CD3 zeta chain is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4B10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD247
Gene ID: 919
Uniprot ID: CD3Z_HUMAN
Specificity: CD247 Monoclonal Antibody detects endogenous levels of CD247 protein.
Immunogen: Purified recombinant fragment of human CD247 expressed in E. Coli.
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN).
Protein Name T-Cell Surface Glycoprotein Cd3 Zeta Chain
T-Cell Receptor T3 Zeta Chain
Cd Antigen Cd247
Database Links Reactome: R-HSA-164944 P20963-1
Reactome: R-HSA-198933 P20963-1
Reactome: R-HSA-202424 P20963-1
Reactome: R-HSA-202427 P20963-1
Reactome: R-HSA-202430 P20963-1
Reactome: R-HSA-202433 P20963-1
Reactome: R-HSA-2029481 P20963-1
Reactome: R-HSA-2029482 P20963-1
Reactome: R-HSA-2029485 P20963-1
Reactome: R-HSA-389948 P20963-1
Reactome: R-HSA-9664323 P20963-1
Reactome: R-HSA-9664422 P20963-1
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Zeta Chain antibody
Anti-T-Cell Receptor T3 Zeta Chain antibody
Anti-Cd Antigen Cd247 antibody
Anti-CD247 antibody
Anti-CD3Z antibody
Anti-T3Z antibody
Anti-TCRZ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance