| Host: | Mouse |
| Applications: | FC |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-B-cell receptor CD22 is suitable for use in Flow Cytometry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | Cy34.1 |
| Conjugation: | PE/Cyanine5 |
| Isotype: | IgG1k |
| Formulation: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Concentration: | 0.2 mg/mL |
| Storage Instruction: | Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
| Gene Symbol: | Cd22 |
| Gene ID: | 12483 |
| Uniprot ID: | CD22_MOUSE |
| Tissue Specificity | B-lymphocytes. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by LYN. Phosphorylation of Tyr-783 and Tyr-843 are involved in binding to SYK. Phosphorylation of Tyr-828 is involved in binding to GRB2. Phosphorylation of Tyr-863 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. |
| Function | Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow. Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration. Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling. If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced. |
| Protein Name | B-Cell Receptor Cd22 B-Lymphocyte Cell Adhesion Molecule Bl-Cam Sialic Acid-Binding Ig-Like Lectin 2 Siglec-2 T-Cell Surface Antigen Leu-14 Cd Antigen Cd22 |
| Database Links | Reactome: R-MMU-198933 Reactome: -MMU-5690714 Reactome: -MMU-983695 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-B-Cell Receptor Cd22 antibody Anti-B-Lymphocyte Cell Adhesion Molecule antibody Anti-Bl-Cam antibody Anti-Sialic Acid-Binding Ig-Like Lectin 2 antibody Anti-Siglec-2 antibody Anti-T-Cell Surface Antigen Leu-14 antibody Anti-Cd Antigen Cd22 antibody Anti-Cd22 antibody Anti-Lyb-8 antibody Anti-Siglec2 antibody |
Information sourced from Uniprot.org