Host: | Goat |
Applications: | WB/IHC-F/IHC-P |
Reactivity: | Human/Rat/Mouse/Monkey/Canine |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-CD19 molecule (510aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunohistochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS only |
Purification: | This antibody is epitope-affinity purified from goat antiserum. |
Concentration: | 3 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC-F 1:250-1:1000 IHC-P 1:250-1:1000 |
Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Gene Symbol: | CD19 |
Gene ID: | 930 |
Uniprot ID: | CD19_HUMAN |
Immunogen Region: | 510aa C-Term |
Accession Number: | ENSG00000177455 |
Specificity: | Using spleen and Jurkat cell lysates detects a 95 kDa band by Western blot. |
Immunogen: | Purified recombinant peptide within residues 510 aa to the C-terminus of human CD19 produced in E. coli. |
Tissue Specificity | Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level). |
Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels. |
Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
Protein Name | B-Lymphocyte Antigen Cd19 B-Lymphocyte Surface Antigen B4 Differentiation Antigen Cd19 T-Cell Surface Antigen Leu-12 Cd Antigen Cd19 |
Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-198933 Reactome: R-HSA-2219530 Reactome: R-HSA-6811558 Reactome: R-HSA-977606 Reactome: R-HSA-983695 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft |
Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibody Anti-B-Lymphocyte Surface Antigen B4 antibody Anti-Differentiation Antigen Cd19 antibody Anti-T-Cell Surface Antigen Leu-12 antibody Anti-Cd Antigen Cd19 antibody Anti-CD19 antibody |
Information sourced from Uniprot.org