Anti-CD19 antibody (422-433) (STJ72998)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CD19 (422-433) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CD19 |
| Gene ID | 930 |
| Uniprot ID | CD19_HUMAN |
| Accession Number | NP_001171569.1; NP_001761.3 |
| Immunogen Region | 422-433 |
| Immunogen Sequence | ENDSNLGQDQLS |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001171569.1; NP_001761.3). |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19B-Lymphocyte Surface Antigen B4Differentiation Antigen Cd19T-Cell Surface Antigen Leu-12Cd Antigen Cd19 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-198933Reactome: R-HSA-2219530Reactome: R-HSA-6811558Reactome: R-HSA-977606Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibodyAnti-B-Lymphocyte Surface Antigen B4 antibodyAnti-Differentiation Antigen Cd19 antibodyAnti-T-Cell Surface Antigen Leu-12 antibodyAnti-Cd Antigen Cd19 antibodyAnti-CD19 antibody |
Information sourced from Uniprot.org