Host: | Rabbit |
Applications: | ELISA/IHC/IP/WB |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-CD19 (200-250) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
Purification: | Affinity Purified |
Concentration: | 0.55-0.75 µg/µl |
Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50-1:150 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Gene Symbol: | CD19 |
Gene ID: | 930 |
Uniprot ID: | CD19_HUMAN |
Immunogen Region: | 200-250 |
Immunogen: | Synthetic peptide taken within amino acid region 200-250 on human CD19 protein. |
Tissue Specificity | Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level). |
Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels. |
Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
Protein Name | B-Lymphocyte Antigen Cd19 B-Lymphocyte Surface Antigen B4 Differentiation Antigen Cd19 T-Cell Surface Antigen Leu-12 Cd Antigen Cd19 |
Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-198933 Reactome: R-HSA-2219530 Reactome: R-HSA-6811558 Reactome: R-HSA-977606 Reactome: R-HSA-983695 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft |
Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibody Anti-B-Lymphocyte Surface Antigen B4 antibody Anti-Differentiation Antigen Cd19 antibody Anti-T-Cell Surface Antigen Leu-12 antibody Anti-Cd Antigen Cd19 antibody Anti-CD19 antibody |
Information sourced from Uniprot.org