![Mouse splenocytes were stained with Anti-CD19 antibody (STJA0000023) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/144876/291546/STJA0000023_1__10014.1631205327.jpg?c=1 "Mouse splenocytes were stained with Anti-CD19 antibody (STJA0000023) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.")
![Mouse splenocytes were stained with Anti-CD19 antibody (STJA0000023) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/144876/291546/STJA0000023_1__10014.1631205327.jpg?c=1 "Mouse splenocytes were stained with Anti-CD19 antibody (STJA0000023) [Used at 0.2 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (blank black histogram) were used as control.")
Anti-Cd19 antibody [1D3] {AF488} (STJA0000023)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationAF488
IsotypeIgG2ak
General Information
| Short Description | Rat monoclonal anti-B-lymphocyte antigen CD19 for use in FC in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1D3 |
| Isotype | IgG2ak |
| Conjugation | AF488 |
| Concentration | 0.5 mg/mL |
| Formulation | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Storage Instruction | Store between 2-8ยฐC and protecting from prolonged exposure to light. Do not freeze this product. |
Target Information
| Gene Symbol | Cd19 |
| Gene ID | 12478 |
| Uniprot ID | CD19_MOUSE |
Additional Info
| Tissue Specificity | Detected on B cells in spleen, bone marrow, thymus and lymph nodes. Detected on peripheral blood lymphocytes (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-522 is extremely rapid, followed by phosphorylation at Tyr-402. In contrast, phosphorylation of Tyr-493 appears more slowly and is more transient, returning rapidly to basal levels. |
| Function | Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Protein Name | B-Lymphocyte Antigen Cd19Differentiation Antigen Cd19Cd Antigen Cd19 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-198933Reactome: -MMU-6811558Reactome: -MMU-977606Reactome: -MMU-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Names | Anti-B-Lymphocyte Antigen Cd19 antibodyAnti-Differentiation Antigen Cd19 antibodyAnti-Cd Antigen Cd19 antibodyAnti-Cd19 antibody |
Information sourced from Uniprot.org