| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD177 antigen (361-410 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CD177 |
| Gene ID: |
57126 |
| Uniprot ID: |
CD177_HUMAN |
| Immunogen Region: |
361-410 aa |
| Specificity: |
CD177 Polyclonal Antibody detects endogenous levels of CD177 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the Internal region of human CD177 at the amino acid range 361-410 |
| Post Translational Modifications | N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). |
| Function | In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis. |
| Protein Name | Cd177 AntigenHuman Neutrophil Alloantigen 2aHna-2aNb1 GlycoproteinNb1 GpPolycythemia Rubra Vera Protein 1Prv-1Cd Antigen Cd177 |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-202733Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorMembrane RaftGpi-Like-AnchorSecretedCytoplasmic Granule MembraneCell ProjectionLamellipodiumCell Surface Expression On Neutrophils Is Increased Upon Tnf-AlphaFmlp Or Cxcl8/Il8-Mediated StimulationIn NeutrophilsStored Predominantly In Secondary And Tertiary GranulesCan Also Be Shedded From The Cell MembraneLocalizes To Lamellar Protrusions In Spreading Neutrophils |
| Alternative Antibody Names | Anti-Cd177 Antigen antibodyAnti-Human Neutrophil Alloantigen 2a antibodyAnti-Hna-2a antibodyAnti-Nb1 Glycoprotein antibodyAnti-Nb1 Gp antibodyAnti-Polycythemia Rubra Vera Protein 1 antibodyAnti-Prv-1 antibodyAnti-Cd Antigen Cd177 antibodyAnti-CD177 antibodyAnti-NB1 antibodyAnti-PRV1 antibodyAnti-UNQ595 antibodyAnti-PRO1181 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
