| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD177 (35-285) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CD177 |
| Gene ID: |
57126 |
| Uniprot ID: |
CD177_HUMAN |
| Immunogen Region: |
35-285 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human CD177 (NP_065139.2). |
| Immunogen Sequence: |
VSDLPRQWTPKNTSCDSGLG CQDTLMLIESGPQVSLVLSK GCTEAKDQEPRVTEHRMGPG LSLISYTFVCRQEDFCNNLV NSLPLWAPQPPADPGSLRCP VCLSMEGCLEGTTEEICPKG TTHCYDGLLRLRGGGIFSNL RVQGCMPQPVCNLLNGTQEI GPVGMTENCDMKDFLTCHRG TTIMTHGNLAQEPTDWTTSN TEMCEVGQVCQETLLLLDVG LTSTLVGTKGCSTVGAQNS |
| Tissue Specificity | Highly expressed in normal bone marrow and weakly expressed in fetal liver. During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level). Expressed by a subset of mature neutrophils (at protein level). The percentage of neutrophils expressing CD177 varies across the population. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). |
| Post Translational Modifications | N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). |
| Function | In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis. |
| Protein Name | Cd177 AntigenHuman Neutrophil Alloantigen 2aHna-2aNb1 GlycoproteinNb1 GpPolycythemia Rubra Vera Protein 1Prv-1Cd Antigen Cd177 |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-202733Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorMembrane RaftGpi-Like-AnchorSecretedCytoplasmic Granule MembraneCell ProjectionLamellipodiumCell Surface Expression On Neutrophils Is Increased Upon Tnf-AlphaFmlp Or Cxcl8/Il8-Mediated StimulationIn NeutrophilsStored Predominantly In Secondary And Tertiary GranulesCan Also Be Shedded From The Cell MembraneLocalizes To Lamellar Protrusions In Spreading Neutrophils |
| Alternative Antibody Names | Anti-Cd177 Antigen antibodyAnti-Human Neutrophil Alloantigen 2a antibodyAnti-Hna-2a antibodyAnti-Nb1 Glycoprotein antibodyAnti-Nb1 Gp antibodyAnti-Polycythemia Rubra Vera Protein 1 antibodyAnti-Prv-1 antibodyAnti-Cd Antigen Cd177 antibodyAnti-CD177 antibodyAnti-NB1 antibodyAnti-PRV1 antibodyAnti-UNQ595 antibodyAnti-PRO1181 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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