• Immunofluorescence analysis of paraffin-embedded Mouse spleen using CD152 Rabbit polyclonal antibody (STJ11105488) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD152 using CD152 Rabbit polyclonal antibody (STJ11105488) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CD152 antibody (36-161) (STJ11105488)

SKU:
STJ11105488

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD152 (36-161) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ctla4
Gene ID: 12477
Uniprot ID: CTLA4_MOUSE
Immunogen Region: 36-161
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 36-161 of mouse CD152 (NP_033973.2).
Immunogen Sequence: EAIQVTQPSVVLASSHGVAS FPCEYSPSHNTDEVRVTVLR QTNDQMTEVCATTFTEKNTV GFLDYPFCSGTFNESRVNLT IQGLRAVDTGLYLCKVELMY PPPYFVGMGNGTQIYVIDPE PCPDSD
Tissue Specificity Widely expressed with highest levels in lymphoid tissues.
Post Translational Modifications N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Protein Name Cytotoxic T-Lymphocyte Protein 4
Cytotoxic T-Lymphocyte-Associated Antigen 4
Ctla-4
Cd Antigen Cd152
Database Links Reactome: R-MMU-389513
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Exists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization
Alternative Antibody Names Anti-Cytotoxic T-Lymphocyte Protein 4 antibody
Anti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibody
Anti-Ctla-4 antibody
Anti-Cd Antigen Cd152 antibody
Anti-Ctla4 antibody
Anti-Cd152 antibody

Information sourced from Uniprot.org

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