Anti-CD141/Thrombomodulin antibody (19-515 aa) [SRM5110] (STJ11106907)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal CD141/Thrombomodulin (19-515 aa) antibody for use in WB, IHC-P, IF, ICC, FC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/FC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5110 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:10000IHC-P:1:100-1:500IF/ICC:1:50-1:200FC:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
Target Information
| Gene Symbol | THBD |
| Gene ID | 7056 |
| Uniprot ID | TRBM_HUMAN |
| Immunogen Region | 19-515 aa |
| Immunogen Sequence | APAEPQPGGSQCVEHDCFAL YPGPATFLNASQICDGLRGH LMTVRSSVAADVISLLLNGD GGVGRRRLWIGLQLPPGCGD PKRLGPLRGFQWVTGDNNTS YSRWARLDLNGAPLCGPLCV AVSAAEATVPSEPIWEEQQC EVKADGFLCEFHFPATCRPL AVEPGAAAAAVSITYGTPFA ARGADFQALPVGSSAAVAPL GLQLMCTAPPGAVQGHWARE APGAWDCSVENGGCEHACN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 19-515 of human CD141/Thrombomodulin. (NP_000352.1). |
Additional Info
| Tissue Specificity | Endothelial cells are unique in synthesizing thrombomodulin. |
| Post Translational Modifications | N-glycosylated. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway. Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition. Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation. Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response. |
| Protein Name | ThrombomodulinTmFetomodulinCd Antigen Cd141 |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-202733 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Thrombomodulin antibodyAnti-Tm antibodyAnti-Fetomodulin antibodyAnti-Cd Antigen Cd141 antibodyAnti-THBD antibodyAnti-THRM antibody |
Information sourced from Uniprot.org