| Host: | Mouse |
| Applications: | IHC/IF/FC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Monocyte differentiation antigen CD14 is suitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5A3 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:200-1:1000 FC 1:200-1:400 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD14 |
| Gene ID: | 929 |
| Uniprot ID: | CD14_HUMAN |
| Specificity: | CD14 Monoclonal Antibody detects endogenous levels of CD14 protein. |
| Immunogen: | Purified recombinant fragment of human CD14 expressed in E. Coli. |
| Post Translational Modifications | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Protein Name | Monocyte Differentiation Antigen Cd14 My23 Antigen Myeloid Cell-Specific Leucine-Rich Glycoprotein Cd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form |
| Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-140534 Reactome: R-HSA-166016 Reactome: R-HSA-166020 Reactome: R-HSA-166058 Reactome: R-HSA-166166 Reactome: R-HSA-168179 Reactome: R-HSA-168188 Reactome: R-HSA-2562578 Reactome: R-HSA-5602498 Reactome: R-HSA-5603041 Reactome: R-HSA-5686938 Reactome: R-HSA-6798695 Reactome: R-HSA-936964 Reactome: R-HSA-937041 Reactome: R-HSA-937072 Reactome: R-HSA-975163 Reactome: R-HSA-9820960 Reactome: R-HSA-9824878 Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Secreted Membrane Raft Golgi Apparatus Secreted Forms May Arise By Cleavage Of The Gpi Anchor |
| Alternative Antibody Names | Anti-Monocyte Differentiation Antigen Cd14 antibody Anti-My23 Antigen antibody Anti-Myeloid Cell-Specific Leucine-Rich Glycoprotein antibody Anti-Cd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form antibody Anti-CD14 antibody |
Information sourced from Uniprot.org