Anti-CD106 antibody (25-310 aa) (STJ11105075)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD106 (25-310 aa) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Vcam1Uniprot ID=VCAM1_MOUSE" |
| Immunogen Region | 25-310 aa |
| Immunogen Sequence | FKIEISPEYKTIAQIGDSMA LTCSTTGCESPLFSWRTQID SPLNAKVRTEGSKSVLTMEP VSFENEHSYLCTATCGSGKL ERSIHVDIYSFPKDPEIQFS GPLEVGKPVTVKCLAPDIYP VYRLEIDLFKGDQLMNRQEF SSEEMTKSLETKSLEVTFTP VIEDIGKALVCRAKLHIDQI DSTLKERETVKELQVYISPR NTTISVHPSTRLQEGGAVTM TCSSEGLPAPEIFWGRKLD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 25-310 of mouse CD106. (NP_035823.3). |
Additional Info
| Tissue Specificity | Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. |
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative Antibody Names | Anti-Vascular Cell Adhesion Protein 1 antibodyAnti-V-Cam 1 antibodyAnti-Vcam-1 antibodyAnti-Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 antibodyAnti-Vcam1 antibodyAnti-Vcam-1 antibody |
Information sourced from Uniprot.org