• Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (2 Mu g/mL, left) or CD10 Rabbit monoclonal antibody (STJ11105672, 2 Mu g/mL, right) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining.
  • Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T cells (right) were surface-stained with CD10 Rabbit monoclonal antibody (STJ11105672, 2 Mu g/mL, orange line) or Rabbit IgG isotype control (2 Mu g/mL, blue line). followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Western blot analysis of lysates from Mouse kidney using CD10 Rabbit monoclonal antibody (STJ11105672) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30 s.
  • Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit monoclonal antibody (STJ11105672) at 1:3000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 60 s.

Anti-CD10 antibody (52-750) [S5672RM] (STJ11105672)

SKU:
STJ11105672

£146.50 - £639.50
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Host: Rabbit
Applications: WB/FC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD10 (52-750) is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5672RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
FC 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mme
Gene ID: 17380
Uniprot ID: NEP_MOUSE
Immunogen Region: 52-750
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of mouse CD10 (NP_032630.2).
Immunogen Sequence: YDDGICKSSDCIKSAARLIQ NMDASVEPCTDFFKYACGGW LKRNVIPETSSRYSNFDILR DELEVILKDVLQEPKTEDIV AVQKAKTLYRSCINESAIDS RGGQPLLKLLPDIYGWPVAS DNWDQTYGTSWTAEKSIAQL NSKYGKKVLINFFVGTDDKN STQHIIHFDQPRLGLPSRDY YECTGIYKEACTAYVDFMIS VARLIRQEQSLPIDENQLSL EMNKVMELEKEIANATTKP
Post Translational Modifications Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Protein Name Neprilysin
Atriopeptidase
Enkephalinase
Neutral Endopeptidase 24.11
Nep
Neutral Endopeptidase
Skin Fibroblast Elastase
Sfe
Cd Antigen Cd10
Database Links Reactome: R-MMU-2022377
Reactome: -MMU-5578768
Reactome: -MMU-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Neprilysin antibody
Anti-Atriopeptidase antibody
Anti-Enkephalinase antibody
Anti-Neutral Endopeptidase 24.11 antibody
Anti-Nep antibody
Anti-Neutral Endopeptidase antibody
Anti-Skin Fibroblast Elastase antibody
Anti-Sfe antibody
Anti-Cd Antigen Cd10 antibody
Anti-Mme antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance