• Western blot analysis of RPMI 8226, using CCR1 rabbit polyclonal antibody (STJ11100297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Rat leg bone using CCR1 rabbit polyclonal antibody (STJ11100297) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse bone marrow using CCR1 rabbit polyclonal antibody (STJ11100297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat bone marrow using CCR1 rabbit polyclonal antibody (STJ11100297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CCR1 antibody (255-354) (STJ11100297)

SKU:
STJ11100297

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCR1 (255-354) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCR1
Gene ID: 1230
Uniprot ID: CCR1_HUMAN
Immunogen Region: 255-354
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 255-354 of human CCR1 (NP_001286.1).
Immunogen Sequence: YNLTILISVFQDFLFTHECE QSRHLDLAVQVTEVIAYTHC CVNPVIYAFVGERFRKYLRQ LFHRRVAVHLVKWLPFLSVD RLERVSSTSPSTGEHELSAG
Tissue Specificity Widely expressed in different hematopoietic cells.
Function Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
Protein Name C-C Chemokine Receptor Type 1
C-C Ckr-1
Cc-Ckr-1
Ccr-1
Ccr1
Hm145
Ld78 Receptor
Macrophage Inflammatory Protein 1-Alpha Receptor
Mip-1alpha-R
Rantes-R
Cd Antigen Cd191
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-C-C Chemokine Receptor Type 1 antibody
Anti-C-C Ckr-1 antibody
Anti-Cc-Ckr-1 antibody
Anti-Ccr-1 antibody
Anti-Ccr1 antibody
Anti-Hm145 antibody
Anti-Ld78 Receptor antibody
Anti-Macrophage Inflammatory Protein 1-Alpha Receptor antibody
Anti-Mip-1alpha-R antibody
Anti-Rantes-R antibody
Anti-Cd Antigen Cd191 antibody
Anti-CCR1 antibody
Anti-CMKBR1 antibody
Anti-CMKR1 antibody
Anti-SCYAR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance